ISSN: 0972-1045

Critical studies on the effect of growth regulators on in vitro shoot proliferation in Rosa x hybrida L. cv. Sonia for micropropagation.

Journal of Applied Horticulture, 1999, 1(2): 91-93.

Critical studies on the effect of growth regulators on in vitro shoot proliferation in Rosa x hybrida L. cv. Sonia for micropropagation.

S.K. Singh and M.M. Syamal

Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, India.

DOI: https://doi.org/10.37855/jah.1999.v01i02.06

Key words: plant growth regulators, in vitro culture, in vitro regeneration, roses, micropropagation, gibberellic acid, IBA, NAA, rooting, acclimatization, roots, ornamental woody plants, shoots, gibberellins, tissue culture, ornamental plants, auxins, cytokinins

Abstract: A micropropagation method for roses cv. Sonia is presented. Shoot proliferation was best (70.3%) on MS [Murashige and Skoog] medium supplemented with BAP [benzyladenine] at 2 mg/litre + NAA at 0.1 mg/litre + GA3 at 0.01 mg/litre, with a proliferation of >5 microshoots per subculture. Efficient rooting was achieved on half-strength MS medium supplemented with IBA at 0.2 mg/litre + NAA at 0.1 mg/litre. Rooted plantlets were acclimatized for 3 weeks and planted out under field conditions with a survival of over 70%.