Scanning electron microscopy analysis of pollen grains of Hibiscus rosa-sinensis cultivars ‘Playboy’ and ‘Gelia Castillo’

S.J. Prejith Raj, A. Sheena*, Beena Thomas, M. Rafeekher and Pratheesh P. Gopinath

Instructional Farm, Kerala Agricultural University, College of Agriculture, Vellayani, Thiruvananthapuram, 695522, Kerala, India. Corresponding e-mail: sheena2sa@yahoo.co.in

DOI: https://doi.org/10.37855/jah.2025.v27i02.29

Key words: Hibiscus, SEM, pollen grains, pollen morphology, exine
Abstract: Pollen morphology of Hibiscus rosa sinensis cultivars ‘Playboy’ and ‘Gelia Castillo’ were studied under light and scanning electron microscope. The two cultivars exhibited spheroidal pollen with pantoporate aperture and spiny surfaces under a Scanning Electron Microscope (SEM). The pollen diameter of the cv ‘Playboy’ and cv ‘Gelia Castillo’ were 116.0 ?m and 129.6 ?m, respectively. The pollen apertures of both the cultivars were similar with round shape but in the cv. ‘Gelia Castillo’, the aperture was partially covered with an exine layer. The cv ‘Gelia Castillo’ exhibited a pollen fertility of 93.84%, whereas the cv ‘Playboy’ exhibited a pollen fertility of 88.57%. This study offers important information regarding the pollen traits of H. rosa sinensis cultivars ‘Playboy’ and ‘Gelia Castillo’. Due to their attractive floral characters and high pollen fertility, both cultivars can be utilized as good male parents in crop improvement programs for breeding novel hibiscus varieties.



Journal of Applied Horticulture