Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Key words: BCG, total alkaloids, medicinal plants, determination
Journal of Applied Horticulture, 2010, volume 12, issue 1, pages 69-70.
Abstract: A simple spectrophotometric method based on the reaction with Bromocresol Green (BCG) was developed for determination of total alkaloids in medicinal plants. A yellow complex forms and is easily extractable by chloroform at pH 4.7. The absorbance of the complex obeys Bear 's law over the concentration range of 4-13 ug atropine per mL of chloroform. This procedure can be carried out in the presence of other compounds without interference.
