Abstract: Pollen studies were conducted in ten single type genotypes of tuberose (Polianthes tuberosa) viz., Shringar (Mexican Single x Pearl Double), Prajwal (Shringar x Mexican Single), Phule Rajani (Mexican Single x Shringar), Calcutta Single, Hyderabad Single, Kahikuchi Single, Mexican Single, Pune Single, Navsari Local and Variegated Single at TNAU, Coimbatore, India. Studies revealed that pollen grains were round in shape, pollen viability in acetocarmine stain was 96.73% in the genotype ‘Variegated Single’ and the germination was maximum (99.21%) in 15% sucrose solution with other chemicals. Pollen tubes grew to a length of 1234.949 microns 24 hours after the dehiscence of anther. Among the genotypes under study, the highest fruit set was 89% under natural open pollination and 0% under artificial self pollination.