Department of Biotechnology, Dr. Y.S. Parmar University of Horticulture and Forestry, Solan, HP, India.
Key words: cauliflowers, callus, cotyledons, explants, in vitro regeneration, kanamycin, in vitro selection, vegetables, biotechnology
Journal of Applied Horticulture, 1999, volume 1, issue 2, pages 94-96.
Abstract: Kanamycin sensitivity studies were conducted to study the resistance level of kanamycin in cauliflower (Brassica oleracea var. botrytis, cv. Pusa Snow Ball). Increasing doses of kanamycin (10, 20, 30, 40, 50 mg/litre) were given to hypocotyl and cotyledon explants to determine a minimum concentration of kanamycin required for selection of putative transformed cells during transformation. Decreases in fresh weight in both cotyledon and hypocotyl tissues were observed with increasing in kanamycin concentration. Even 50 mg/litre kanamycin did not completely inhibit the growth but callus formation and shoot regeneration was affected. It is suggested that at least 20-30 mg/litre kanamycin would be necessary to select resistant transformants in callus and shoot cultures.
