Abstract: Earlier studies have shown that the degree of success at each stage of micropropagation in grapevine is genotype dependent; hence it becomes imperative to optimize culture conditions for rapid propagation of a variety. Present report describes two approaches of in vitro propagation of a Vitis vinifera cultivar, Red Globe. In one approach, whole plants could be developed from single node segments by bud break and direct rooting in vitro. Eight different basal media tried showed different morphogenetic responses. In second approach, multiple shoots were induced in nodal segments cultured on MS basal medium supplemented with BA (8.88 uM). Also, second crop of shoots could be induced in left over nodal segments devoid of shoots. Rooting of shoots could be induced in vitro, both in semi-solid or liquid media and also ex vitro by pulse treatment of IAA (2.85 uM) + NAA (2.70 uM). Plant establishment in later case was 80%. A simple procedure described here can complement conventional methods, currently being used in propagation of this important grape variety.