In vitro micropropagation of Ananas comosus L. (Merr.) var.
Queen.
Mandal, A. B. , Aparna Maiti , Elanchezhian, R.
Plant Genetics and Biotechnology Division, Central Agricultural
Research Institute, Port Blair - 744 101, Andamans, India.
Axillary buds from crown of mature fruits, slips and suckers of
pineapple cv. Queen cultured in MS medium supplemented with high
auxin:cytokin ratio and then transferred to MS medium with high
cytokinin:auxin ratio showed high establishment percentage. Shoot
multiplication increased upon subculture on freshly prepared MS medium
supplemented with benzyladenine (BA). The best multiplication medium
was an MS medium supplemented with 2.5 mg BA/litre, 2 mg NAA and
IBA/litre and 10% coconut water. In vitro grown shootlets were
successfully rooted in MS medium supplemented with 0.5 mg NAA and
IBA/litre. The highest number of buds produced in 6 months was obtained
from shaken liquid medium. Pulsing of explants enhanced the culture
response as indicated by higher shoot multiplication rate in all types
of explants. |
