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Plant regeneration from zygotic embryo
hypocotyls of Tunisian chili (Capsicum annuum L.).
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| Arous,
S.;Boussaïd, M.;Marrakchi, M |
| Faculté des
Sciences de Tunis, Campus Universitaire, Tunis, Tunisia. |
| This paper
reports the regeneration of Tunisian pepper cultivar from zygotic
embryos cultured in vitro . Zygotic embryos of the Tunisian cultivar
D'hirat cultured in vitro developed adventitious buds. The best results
for bud induction were obtained in the Murashige and Skoog medium,
supplemented with 5 mg benzylaminopurine (BA)/litre and 1 mg NAA/litre.
The important effect of BA in adventitious bud formation was
demonstrated. Shoot bud development was enhanced by the addition of
gibberellic acid to the medium. Plants were rooted in half-strength
Murashige and Skoog medium and transferred into pots, containing loam.
To test the stability of the regenerants, characters related to the
fertility, fruit quality, leaf and flower were measured. Regenerants and
their progeny were compared to the control plants derived from seeds.
Variance analysis and CANDISC were used. No significant differences
were detected between the regenerants and the control plants for the
characters tested. . |
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