Enhancing
micropropagation efficiency of strawberry using bandage in liquid media |
Neeru
Sood,Ranjan Srivastava,Singh, O. S.,Gosal, S. S |
Biotechnology Center, Punjab Agricultural University, Ludhiana - 141
004, India. |
The rate of
strawberry propagation through conventional technique is quite low and
it is difficult to maintain plant material during the summer months. In
the present investigation, the protocol for tissue culture propagation
has been further improved by using liquid medium with four layers of
surgical bandage. Fifteen milliliters of liquid Murashige and Skoog's
(MS) medium enriched with BAP [benzyladenine] and Kinetin was used for
the study. The proliferating shoots were longer, thicker, and borne with
broader leaves in liquid medium. Studies on the survival of such plants
were also conducted. The survival rate on various substrates varied
from 66.67% in soil to 76.67% in soilrite mix. Seventy percent of
plantlets transferred on sand survived well. |
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