| In vitro
plant regeneration of Citrus aurantifolia through callus culture |
| Srivastava,
R. K.,Sandhu, A. S.,Neeru Sood |
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Department of Horticulture, Punjab Agricultural University, Ludhiana -
141 004, India. |
|
Callus was induced from different explants of in vitro raised seedlings
of C. aurantifolia to study the response of explants and medium
composition on frequency of callusing and to develop reliable protocol
for high frequency of plant regeneration from callus cultures. Shoot
tip, epicotyl and hypocotyl were found superior explants for callusing
in terms of amount of callusing, days to callus and callus induction
frequency. Addition of cytokinin was found indispensable for
regeneration and MS medium enriched with BAP [benzyladenine] (5 mg/l)
resulted in highest per cent of callus regeneration. Regenerants were
rooted in vitro and hardened in plastic pots containing autoclaved soil. |
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