Litchi shoot
bud culture for micropropagation |
Chandra,
R.,Padaria, J. C |
Central Institute for Subtropical Horticulture, R.B. Road, P.O.
Dilkusha, Lucknow-226 002, India. |
In
experiments to establish shoot bud culture techniques for litchi (Litchi
chinensis ) cv. Seedless, the problems of microbial contamination,
phenol exudation and explant browning were controlled to a great extent
by using mercuric chloride or sodium hypochlorite. Culturing of shoot
buds in L6 medium (MS + 0.2 mg BA + 0.5 mg GA3 + 0.1 mg IAA/litre)
resulted in shoot differentiation and growth. Maximum response was
obtained in May-June. |
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